Spectra™ 10 Plus Lyme vaccine is a combination of immunogenic, attenuated strains of CDV, CAV2, CPIV, and CPV type 2b, propagated in cell line tissue cultures. The diluent contains killed CCV and Bburg antigens with Leptospira bacterial antigens. The CAV2 fraction has been shown to be effective against disease due to CAV1. The CPV2b strain has been demonstrated to be effective against CPV2c in puppies with CPV maternal antibody. Gentamicin and thimerosal added as preservatives.